Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release
Copyright policy )Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release
Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins--selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on gamma-aminobutyric acid type A (GABAA) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.
- Rutgers New Jersey Medical School United States
- Rutgers, The State University of New Jersey United States
- McGill University Canada
- Rutgers Health United States
Neurons, Patch-Clamp Techniques, Quinpirole, Dose-Response Relationship, Drug, Dopamine, Dose-Response Relationship, Radiation, Neural Inhibition, Enkephalin, Ala(2)-MePhe(4)-Gly(5)-, In Vitro Techniques, Bicuculline, Electric Stimulation, Membrane Potentials, Rats, GABA Antagonists, Animals, Newborn, Mesencephalon, Dopamine Agonists, Animals, Humans, Glial Cell Line-Derived Neurotrophic Factor
Neurons, Patch-Clamp Techniques, Quinpirole, Dose-Response Relationship, Drug, Dopamine, Dose-Response Relationship, Radiation, Neural Inhibition, Enkephalin, Ala(2)-MePhe(4)-Gly(5)-, In Vitro Techniques, Bicuculline, Electric Stimulation, Membrane Potentials, Rats, GABA Antagonists, Animals, Newborn, Mesencephalon, Dopamine Agonists, Animals, Humans, Glial Cell Line-Derived Neurotrophic Factor
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