Determination of lymphoid cell fate is dependent on the expression status of the IL-7 receptor
Copyright policy )Determination of lymphoid cell fate is dependent on the expression status of the IL-7 receptor
Signaling through the IL-7 receptor (IL-7R) is necessary for the development of the earliest B- and T-lineage cells. IL-7R is first expressed on common lymphoid progenitor cells and is not detected on primitive common myeloid progenitors. In this study, we show that enforced expression of IL-7R on multipotential stem cells does not influence lymphoid versus myeloid cell fate. T cell development was compatible with sustained IL-7R expression; however, we observed a near complete block in B cell development at the onset of B-lineage commitment. Unlike pre-proB cells from control animals, developmentally-arrested IL-7R(+)B220(+)CD19(-)NK1.1(-)Ly-6C(-) cells failed to express EBF and Pax5. These results suggest that transient downregulation of IL-7R signaling is a necessary event for induction of EBF and Pax5 expression and B-lymphocyte commitment.
- University of Alabama at Birmingham United States
- Alabama Agricultural and Mechanical University United States
B-Lymphocytes, Receptors, Interleukin-7, Base Sequence, Interleukin-6, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, PAX5 Transcription Factor, Down-Regulation, Bone Marrow Cells, Cell Line, DNA-Binding Proteins, Mice, Inbred C57BL, Mice, Animals, Transplantation, Homologous, Fluorouracil, Lymphocytes, DNA Primers, Stem Cell Transplantation, Transcription Factors
B-Lymphocytes, Receptors, Interleukin-7, Base Sequence, Interleukin-6, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, PAX5 Transcription Factor, Down-Regulation, Bone Marrow Cells, Cell Line, DNA-Binding Proteins, Mice, Inbred C57BL, Mice, Animals, Transplantation, Homologous, Fluorouracil, Lymphocytes, DNA Primers, Stem Cell Transplantation, Transcription Factors
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