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SAYP and Brahma are important for ‘repressive’ and ‘transient’ Pol II pausing

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 24 May 2012 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 40, pages 7,319-7,331 (issn: 0305-1048, eissn: 1362-4962,

Authors: J. V. Nikolenko; Nadezhda E. Vorobyeva; Sofia G. Georgieva; Elena N. Nabirochkina; Yulii V. Shidlovskii; A. N. Krasnov;

doi: 10.1093/nar/gks472

pmid: 22638575

pmc: PMC3424582

SAYP and Brahma are important for ‘repressive’ and ‘transient’ Pol II pausing

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Abstract

Drosophila SAYP, a homologue of human PHF10/BAF45a, is a metazoan coactivator associated with Brahma and essential for its recruitment on the promoter. The role of SAYP in DHR3 activator-driven transcription of the ftz-f1 gene, a member of the ecdysone cascade was studied. In the repressed state of ftz-f1 in the presence of DHR3, the Pol II complex is pre-recruited on the promoter; Pol II starts transcription but is paused 1.5 kb downstream of the promoter, with SAYP and Brahma forming a 'nucleosomal barrier' (a region of high nucleosome density) ahead of paused Pol II. SAYP depletion leads to the removal of Brahma, thereby eliminating the nucleosomal barrier. During active transcription, Pol II pausing at the same point correlates with Pol II CTD Ser2 phosphorylation. SAYP is essential for Ser2 phosphorylation and transcription elongation. Thus, SAYP as part of the Brahma complex participates in both 'repressive' and 'transient' Pol II pausing.

Related Organizations

Russian Academy of Sciences
Russian Federation
Institute of Gene Biology
Russian Federation

Keywords

Transcription, Genetic, Receptors, Cytoplasmic and Nuclear, Cell Cycle Proteins, Gene Regulation, Chromatin and Epigenetics, Chromatin, Cell Line, Nucleosomes, DNA-Binding Proteins, Gene Expression Regulation, Serine, Trans-Activators, Animals, Drosophila Proteins, Drosophila, RNA Polymerase II, Transcription Initiation Site, Promoter Regions, Genetic, Transcription Factors

16 Research products, page 1 of 2

Ftz transcription factor 1, isoform D
2017IsRelatedTo
Probable nuclear hormone receptor HR3
2017IsRelatedTo
Brahma, isoform F
2017IsRelatedTo
Enhancer of yellow 3, isoform D
2017IsRelatedTo
Enhancer of yellow 3, isoform B
2017IsRelatedTo
Nuclear hormone receptor FTZ-F1
2017IsRelatedTo
Enhancer of yellow 3, isoform C
2017IsRelatedTo
ATP-dependent helicase brm
2017IsRelatedTo
Hormone receptor 3, isoform H
2018IsRelatedTo
Hormone receptor 3, isoform F
2018IsRelatedTo

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