Insulin receptor substrate p53 (IRSp53) is a key player in cytoskeletal dynamics, interacting with the actin modulators WAVE2 and Mena. Here, we identified a PDZ protein, MALS, as an IRSp53‐interacting protein using a yeast two‐hybrid screen. A pull‐down assay showed that IRSp53 and MALS interact through the PDZ domain of MALS and the C‐terminal PDZ‐binding sequence of IRSp53. Their interaction in MDCK cells was also demonstrated by co‐immunoprecipitation. Immunocytochemistry showed the colocalization of IRSp53 and MALS at cell–cell contacts. Cytochalasin D induced the redistribution of both proteins to the cytosol. Thus, MALS is a partner of IRSp53 anchoring the actin‐based membrane cytoskeleton at cell–cell contacts.