Cloning and sequencing of the cDNA encoding the avian kidney cAMP-dependent protein kinase inhibitor protein
Copyright policy )Cloning and sequencing of the cDNA encoding the avian kidney cAMP-dependent protein kinase inhibitor protein
The endogenous inhibitor of cAMP-dependent protein kinase (PKA) is down-regulated in the kidneys from vitamin-D-replete chicks as compared to vitamin-D-deficient chicks. Screening of a vitamin-D-deficient chick kidney library resulted in the isolation of a 450-bp cDNA clone encoding the 76-amino acid (aa) protein kinase inhibitor (PKI). The deduced aa sequence of avian PKI shares 80 and 41% identity with the mammalian PKI alpha and PKI beta 1 isoforms, respectively. The chick and mammalian PKI contain conserved N-terminal sequences, including the pseudo-substrate site (18GRRNA22), which are required for potent inhibition of the catalytic subunit of PKA. Chick kidney PKI contains ten unique aa in the C-terminal portion of the protein that are not shared with the mammalian PKI alpha or beta isoforms.
- University of California System United States
- University of California, Riverside United States
DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Sequence Analysis, DNA, Kidney, Cyclic AMP-Dependent Protein Kinases, Animals, Amino Acid Sequence, Cloning, Molecular, Carrier Proteins, Chickens
DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Sequence Analysis, DNA, Kidney, Cyclic AMP-Dependent Protein Kinases, Animals, Amino Acid Sequence, Cloning, Molecular, Carrier Proteins, Chickens
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