Additional file 8: Figure S8. Schematic representation of the pro-resilin gene and its products. (A) The full-length Pro-Resilin protein has 620 residues. It has three functional domains. Following the signal peptide (SP, white box) in the N-terminus, there are a number of tandem repeats (type A repeats, dark grey boxes), followed by an R&R-2 chitin-binding domain, which precedes an array of tandem repeats (type B repeats, light grey boxes). A shorter isoform lacks the R&R-2 domain and has 575 residues (not shown). Deletion of 3 bases in the second exon coding for the R&R-2 domain results in the transversion of the M365 to an I and the deletion of R366 as shown above the full-length Pro-Resilin protein (Crispr/Cas92). Deletion of the cytosine 35 and adenosine 36 of the ORF causes a frameshift of the ORF resulting in a premature stop codon after 22 residues (Pro-ResilinCC-ATG). Information on the mutations at the DNA level is presented in Additional file 10: Figure S10. (B) The pro-resilin gene is composed of three exons separated by two introns. Exon 1 and 3 can be spliced together omitting exon 2 that encodes the R&R-2 domain. The recognition sites of hairpin RNAs against pro-resilin expression are indicated below the scheme. (C) The GFP coding region is inserted in frame into the 5′ end of the pro-resilin gene just before the stop codon, thereby disrupting the recognition site of hairpin RNAs.