Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1α and eEF1A-2/S1 in the central nervous system during mouse development
Copyright policy )Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1α and eEF1A-2/S1 in the central nervous system during mouse development
During early postnatal development, a switch occurs between eEF1A-1/EF-1alpha and eEF1A-2/S1, homologous peptide elongation factors, in brain, heart, and skeletal muscle; eEF1A-2/S1 becomes the major form expressed in maturity. By immunofluorescent labeling, we detected both homologues in the developing brains of wild-type and wasted mutant mice, carrying a deletion in the eEF1A-2/S1 gene; we found that brain expression of eEF1A-2/S1 protein is restricted to mature, terminally differentiated neurons, and coincides with the disappearance of eEF1A-1/EF-1alpha 20 days after birth. Furthermore, no elongation factor 1A is present in wasted mutant mice neurons following the developmental switch, indicating that the genetic regulation silencing eEF1A-1/EF-1alpha is still functional.
- University of Louisville United States
- McGill University Canada
Central Nervous System, Neurons, Heterozygote, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Fluorescent Antibody Technique, Gene Expression Regulation, Developmental, Embryo, Mammalian, Mice, Mutant Strains, Mice, Inbred C57BL, Mice, Peptide Elongation Factor 1, Animals, Newborn, Neurofilament Proteins, Glial Fibrillary Acidic Protein, Mutation, Animals, RNA, Messenger
Central Nervous System, Neurons, Heterozygote, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Fluorescent Antibody Technique, Gene Expression Regulation, Developmental, Embryo, Mammalian, Mice, Mutant Strains, Mice, Inbred C57BL, Mice, Peptide Elongation Factor 1, Animals, Newborn, Neurofilament Proteins, Glial Fibrillary Acidic Protein, Mutation, Animals, RNA, Messenger
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