Serum amylase isozyme of various breeds of domestic fowl and four species of jungle fowl was studied from genetico-biochemical aspects. The results are summarized as follows:1) In respect of serum amylase isozyme of domestic fowl, 5 bands were detected on the anode side and among these bands, genetic variations were seen in the bands A and B and the existence of three phenotypes, AA, AB and BB was confirmed. From the results of various mating experiments, these bands A and B were found to be controlled by a pair of co-dominant alleles, Amy-1A and Amy-1B located on autosome.2) As to various breeds of domestic fowl classified per origin or use, frequency of phenotype and gene frequency of serum amylase isozyme were examined. As a result, distinct differences among breeds were observed in the frequency of occurrence. The above results seem to be suggestive of the possibility of studies of breed differentiation in domestic fowl with genes controlling amylase isozyme as markers.3) Serum amylase isozyme of four species of jungle fowl, Gallus gallus, Gallus sonneratii, Gallus varius and Gallus lafayetti was analyzed and compared with that of domestic fowl. As a result, coincidence of the phenotypes of serum amylase isozyme of these jungle fowl with those of domestic fowl was confirmed.