Copyright information:Taken from "Role of human in breast cancer"http://breast-cancer-research.com/content/9/5/R58Breast cancer research : BCR 2007;9(5):R58-R58.Published online 10 Sep 2007PMCID:PMC2242655. RT-PCR for HGFIN and GAPDH in non-tumorigenic (NT) (MCF10 and MCF12A) and tumorigenic (T) (DU4475) breast cells. P1, P10 and P38 are the non-identifier codes for patients. Total RNA was analyzed by northern analyses for HGFIN from MCF12A non-tumorigenic cells and tumorigenic cell lines (HCC70 and T47D). Membranes were stripped and re-probed for 18S rRNA. and Representative hybridizations for HGFIN from benign breast tissue, = 25 and malignant cells, = 50 . Arrow shows dense staining for alkaline phosphatase (blue) in the benign section. The images were acquired with 100×/0.3 NA objectives.