Involvement of the PP2C-Like Phosphatase Ptc2p in the DNA Checkpoint Pathways of Saccharomyces cerevisiae
Copyright policy )Involvement of the PP2C-Like Phosphatase Ptc2p in the DNA Checkpoint Pathways of Saccharomyces cerevisiae
Abstract RAD53 encodes a conserved protein kinase that acts as a central transducer in the DNA damage and the DNA replication checkpoint pathways in Saccharomyces cerevisiae. To identify new elements of these pathways acting with or downstream of RAD53, we searched for genes whose overexpression suppressed the toxicity of a dominant-lethal form of RAD53 and identified PTC2, which encodes a protein phosphatase of the PP2C family. PTC2 overexpression induces hypersensitivity to genotoxic agents in wild-type cells and is lethal to rad53, mec1, and dun1 mutants with low ribonucleotide reductase activity. Deleting PTC2 specifically suppresses the hydroxyurea hypersensitivity of mec1 mutants and the lethality of mec1Δ. PTC2 is thus implicated in one or several functions related to RAD53, MEC1, and the DNA checkpoint pathways.
Saccharomyces cerevisiae Proteins, Base Sequence, Cell Cycle Proteins, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Substrate Specificity, Protein Phosphatase 2C, Checkpoint Kinase 2, Ribonucleotide Reductases, Phosphoprotein Phosphatases, Genes, Lethal, Protein Phosphatase 2, DNA, Fungal, Protein Kinases, Alleles, DNA Primers
Saccharomyces cerevisiae Proteins, Base Sequence, Cell Cycle Proteins, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Substrate Specificity, Protein Phosphatase 2C, Checkpoint Kinase 2, Ribonucleotide Reductases, Phosphoprotein Phosphatases, Genes, Lethal, Protein Phosphatase 2, DNA, Fungal, Protein Kinases, Alleles, DNA Primers
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