Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton
Copyright policy )Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton
The yeast tropomyosin gene, designated TPM1, is present in a single copy per haploid genome and encodes a protein with a predicted molecular weight of 23.5 kd. The protein sequence is homologous to higher cell tropomyosins, including the characteristic hydrophobic-hydrophilic pseudoheptapeptide repeats. Indirect immunofluorescence microscopy reveals that tropomyosin is localized with actin cables in wild-type cells. Disruption of TPM1 is not lethal, but results in a reduced growth rate and disappearance of actin cables. Strains carrying the conditional actin mutation act1-2 also lack actin cables; overexpression of tropomyosin in these strains partially restores actin cables. These results strongly suggest that tropomyosin interacts with F actin in vivo and may play an important role in assembling or stabilizing actin cables in yeast.
- Cornell University United States
Base Sequence, Gene Expression Regulation, Genes, Molecular Sequence Data, Genes, Lethal, Saccharomyces cerevisiae, Tropomyosin, Actins, Cytoskeleton
Base Sequence, Gene Expression Regulation, Genes, Molecular Sequence Data, Genes, Lethal, Saccharomyces cerevisiae, Tropomyosin, Actins, Cytoskeleton
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