Expression of the DAL80 gene, whose product is homologous to the GATA factors and is a negative regulator of multiple nitrogen catabolic genes in Saccharomyces cerevisiae, is sensitive to nitrogen catabolite repression.
Copyright policy )Expression of the DAL80 gene, whose product is homologous to the GATA factors and is a negative regulator of multiple nitrogen catabolic genes in Saccharomyces cerevisiae, is sensitive to nitrogen catabolite repression.
We have cloned the negative regulatory gene (DAL80) of the allantoin catabolic pathway, characterized its structure, and determined the physiological conditions that control DAL80 expression and its influence on the expression of nitrogen catabolic genes. Disruption of the DAL80 gene demonstrated that it regulates multiple nitrogen catabolic pathways. Inducer-independent expression was observed for the allantoin pathway genes DAL7 and DUR1,2, as well as the UGA1 gene required for gamma-aminobutyrate catabolism in the disruption mutant. DAL80 transcription was itself highly sensitive to nitrogen catabolite repression (NCR), and its promoter contained 12 sequences homologous to the NCR-sensitive UASNTR. The deduced DAL80 protein structure contains zinc finger and coiled-coil motifs. The DAL80 zinc finger motif possessed high homology to the transcriptional activator proteins required for expression of NCR-sensitive genes in fungi and the yeast GLN3 gene product required for functioning of the NCR-sensitive DAL UASNTR. It was also homologous to the three GATAA-binding proteins reported to be transcriptional activators in avian and mammalian tissues. The latter correlations raise the possibility that both positive and negative regulators of allantoin pathway transcription may bind to similar sequences.
- University of Tennessee System United States
- University of Tennessee Health Science Center United States
Base Sequence, Nitrogen, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, Blotting, Northern, GATA Transcription Factors, Fungal Proteins, Ligases, Repressor Proteins, Kinetics, Phenotype, Gene Expression Regulation, Fungal, Sequence Homology, Nucleic Acid, Mutation, Genes, Regulator, Carbon-Nitrogen Ligases, Amino Acid Sequence, Allantoin, Cloning, Molecular, DNA, Fungal
Base Sequence, Nitrogen, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, Blotting, Northern, GATA Transcription Factors, Fungal Proteins, Ligases, Repressor Proteins, Kinetics, Phenotype, Gene Expression Regulation, Fungal, Sequence Homology, Nucleic Acid, Mutation, Genes, Regulator, Carbon-Nitrogen Ligases, Amino Acid Sequence, Allantoin, Cloning, Molecular, DNA, Fungal
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).148 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!