Dynamic Regulation of Transcription Factors by Nucleosome Remodeling
Copyright policy )Dynamic Regulation of Transcription Factors by Nucleosome Remodeling
The chromatin landscape and promoter architecture are dominated by the interplay of nucleosome and transcription factor (TF) binding to crucial DNA sequence elements. However, it remains unclear whether nucleosomes mobilized by chromatin remodelers can influence TFs that are already present on the DNA template. In this study, we investigated the interplay between nucleosome remodeling, by either yeast ISW1a or SWI/SNF, and a bound TF. We found that a TF serves as a major barrier to ISW1a remodeling, and acts as a boundary for nucleosome repositioning. In contrast, SWI/SNF was able to slide a nucleosome past a TF, with concurrent eviction of the TF from the DNA, and the TF did not significantly impact the nucleosome positioning. Our results provide direct evidence for a novel mechanism for both nucleosome positioning regulation by bound TFs and TF regulation via dynamic repositioning of nucleosomes.
- Howard Hughes Medical Institute United States
- Southern Illinois University School of Medicine United States
- Cornell University United States
- Johns Hopkins University United States
Saccharomyces cerevisiae Proteins, Optical Tweezers, QH301-705.5, Chromosomal Proteins, Non-Histone, Science, Biophysics, Biochemistry, Models, Biological, chromatin remodeling, Biology (General), transcription factor, single molecule studies, Adenosine Triphosphatases, Q, R, DNA, Chromatin Assembly and Disassembly, Nucleosomes, DNA-Binding Proteins, optical trapping, Medicine, gene regulation, Plasmids, Transcription Factors
Saccharomyces cerevisiae Proteins, Optical Tweezers, QH301-705.5, Chromosomal Proteins, Non-Histone, Science, Biophysics, Biochemistry, Models, Biological, chromatin remodeling, Biology (General), transcription factor, single molecule studies, Adenosine Triphosphatases, Q, R, DNA, Chromatin Assembly and Disassembly, Nucleosomes, DNA-Binding Proteins, optical trapping, Medicine, gene regulation, Plasmids, Transcription Factors
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