A study on the effect of different doses of Ovulin hormone suspended in saline on the breeding performance of Clarias anguillaris and Clarias gariepinus was carried out. The experiment was conducted in a 2x5 factorial experiment in a Completely Randomized Design at the Hatchery Unit of the Department of Fisheries and Aquaculture, Usmanu Danfodiyo University, Sokoto. Species and hormone dilutions constituted the factors with specie having 2 levels (C. anguillaris and C. gariepinus) and Ovulin suspended in saline at 5 levels (0%, 25%, 50%, 75% and 100%). The result showed that species levels did not significantly (P>0.05) affect the breeding performance in all the breeding performance parameters observed. However, fertilization rate, hatching rate and survival rate were significantly affected (P<0.05) by different levels of Ovulin suspended in saline, but did not have significant influence (P>0.05) on egg weight, spawning fecundity and relative fecundity. It could be concluded from this study that Ovulin suspended in saline can have significant influence on the breeding performance of African catfish.