The chromatin remodelling complex WSTF–SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription
Copyright policy )The chromatin remodelling complex WSTF–SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription
Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein–protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference‐mediated knockdown of NM1 and WSTF reduced pre‐rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre‐assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.
- Stockholm University Sweden
- Karolinska Institute Sweden
- University of Würzburg Germany
- German Cancer Research Center Germany
Adenosine Triphosphatases, Cell Nucleus, Transcription, Genetic, Chromosomal Proteins, Non-Histone, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Chromatin Assembly and Disassembly, Myosin Type I, RNA Polymerase I, Humans, HeLa Cells, Protein Binding, Transcription Factors
Adenosine Triphosphatases, Cell Nucleus, Transcription, Genetic, Chromosomal Proteins, Non-Histone, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Chromatin Assembly and Disassembly, Myosin Type I, RNA Polymerase I, Humans, HeLa Cells, Protein Binding, Transcription Factors
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