YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex
Copyright policy )YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex
In Saccharomyces cerevisiae the pyruvate dehydrogenase (PDH) complex is regulated by reversible phosphorylation of its Pda1p subunit. We here provide evidence that Pda1p is phosphorylated by the mitochondrial kinase Yil042cp. Deletion of YOR090c, encoding a putative mitochondrial phosphatase, results in a decreased PDH activity, indicating that Yor090cp acts as the corresponding PDH phosphatase. We demonstrate by means of blue native gel electrophoresis and tandem affinity purification that both enzymes are associated with the PDH complex.
- TU Dresden Germany
- University of Konstanz Germany
Saccharomyces cerevisiae Proteins, Molecular Sequence Data, S. cerevisiae, Pyruvate Dehydrogenase Complex, YOR090c, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Mitochondrial Proteins, Pyruvate dehydrogenase complex, Humans, Amino Acid Sequence, Phosphorylation, Conserved Sequence, Sequence Homology, Amino Acid, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, Phosphoproteins, Mitochondria, Protein Subunits, Pyruvate Dehydrogenase (Lipoamide)-Phosphatase, YIL042c, Protein Kinases, Sequence Alignment, Protein Binding
Saccharomyces cerevisiae Proteins, Molecular Sequence Data, S. cerevisiae, Pyruvate Dehydrogenase Complex, YOR090c, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Mitochondrial Proteins, Pyruvate dehydrogenase complex, Humans, Amino Acid Sequence, Phosphorylation, Conserved Sequence, Sequence Homology, Amino Acid, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, Phosphoproteins, Mitochondria, Protein Subunits, Pyruvate Dehydrogenase (Lipoamide)-Phosphatase, YIL042c, Protein Kinases, Sequence Alignment, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).50 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!