A Novel Colonic Repressor Element Regulates Intestinal Gene Expression by Interacting with Cux/CDP
Copyright policy )A Novel Colonic Repressor Element Regulates Intestinal Gene Expression by Interacting with Cux/CDP
Intestinal gene regulation involves mechanisms that direct temporal expression along the vertical and horizontal axes of the alimentary tract. Sucrase-isomaltase (SI), the product of an enterocyte-specific gene, exhibits a complex pattern of expression. Generation of transgenic mice with a mutated SI transgene showed involvement of an overlapping CDP (CCAAT displacement protein)-GATA element in colonic repression of SI throughout postnatal intestinal development. We define this element as CRESIP (colon-repressive element of the SI promoter). Cux/CDP interacts with SI and represses SI promoter activity in a CRESIP-dependent manner. Cux/CDP homozygous mutant mice displayed increased expression of SI mRNA during early postnatal development. Our results demonstrate that an intestinal gene can be repressed in the distal gut and identify Cux/CDP as a regulator of this repression during development.
- The University of Texas Southwestern Medical Center United States
- University of Pennsylvania United States
Homeodomain Proteins, Mice, Knockout, Aging, Colon, Gene Expression Regulation, Developmental, Nuclear Proteins, Mice, Transgenic, GATA4 Transcription Factor, DNA-Binding Proteins, Intestines, Mice, COS Cells, Intestine, Small, Mutagenesis, Site-Directed, Animals, Humans, Caco-2 Cells, Intestinal Mucosa, Promoter Regions, Genetic, In Situ Hybridization
Homeodomain Proteins, Mice, Knockout, Aging, Colon, Gene Expression Regulation, Developmental, Nuclear Proteins, Mice, Transgenic, GATA4 Transcription Factor, DNA-Binding Proteins, Intestines, Mice, COS Cells, Intestine, Small, Mutagenesis, Site-Directed, Animals, Humans, Caco-2 Cells, Intestinal Mucosa, Promoter Regions, Genetic, In Situ Hybridization
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