SECRET AGENT, an Arabidopsis thaliana O‐GlcNAc transferase, modifies the Plum pox virus capsid protein
Copyright policy )SECRET AGENT, an Arabidopsis thaliana O‐GlcNAc transferase, modifies the Plum pox virus capsid protein
The capsid protein of Plum pox virus (PPV‐CP) is modified with O‐linked GlcNAc (O‐GlcNAc). While Arabidopsis has two O‐GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV‐CP and that the modification plays a role in the infection process. Here, we show that when co‐expressed in Escherichia coli SEC modifies PPV‐CP. Deletion mapping and site‐directed mutagenesis identified three threonine and a serine located near the N‐terminus of PPV‐CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.
- University of Minnesota System United States
- Southwest Biological Science Center United States
- University of Minnesota Crookston United States
- Spanish National Research Council Spain
- AUS (United States) United States
Threonine, Glycosylation, Arabidopsis thaliana, Capsid protein, Arabidopsis, N-Acetylglucosaminyltransferases, Serine, Sequence Deletion, Binding Sites, Plum pox virus, Base Sequence, Arabidopsis Proteins, Recombinant Proteins, Amino Acid Substitution, Plum Pox Virus, O-GlcNAc transferase, DNA, Viral, O-GlcNAc, Mutagenesis, Site-Directed, Capsid Proteins, Protein Processing, Post-Translational, SECRET AGENT
Threonine, Glycosylation, Arabidopsis thaliana, Capsid protein, Arabidopsis, N-Acetylglucosaminyltransferases, Serine, Sequence Deletion, Binding Sites, Plum pox virus, Base Sequence, Arabidopsis Proteins, Recombinant Proteins, Amino Acid Substitution, Plum Pox Virus, O-GlcNAc transferase, DNA, Viral, O-GlcNAc, Mutagenesis, Site-Directed, Capsid Proteins, Protein Processing, Post-Translational, SECRET AGENT
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