Cloning of the Rat IL-5Rα Gene: Analysis of 5′-Upstream Region and Expression by B Cells
Copyright policy )Cloning of the Rat IL-5Rα Gene: Analysis of 5′-Upstream Region and Expression by B Cells
Although rats are widely used for the analysis of allergic reactions and parasitic infections where IL-5 is involved, nothing is currently known of the expression of IL-5 receptor in this species. In this study, the cDNA sequence, genomic structure and the transcriptional regulation of the rat IL-5Ralpha were analyzed. The rat IL-5Ralpha gene, which we localized to chromosome 4q34-q41, spans more than 25 kb and consists of 12 exons. Promoter activity was seen in different cell lines and analysis by deletion experiments allowed to identify two negative regulatory regions which did not differ when tested either with IL-5Ralpha-negative or positive cells. Finally, the investigation of the expression of IL-5Ralpha showed that it is expressed in lung, spleen, liver, and purified rat B cells from normal rat. This can provide an explanation for the role of rat IL-5 as B-cell growth factor and a relevant model in order to better understand the activity of IL-5 on human B cells.
- French National Centre for Scientific Research France
- Université Libre de Bruxelles Belgium
- Pasteur Institute of Lille France
- Institut Pasteur France
- Institut de Biologie de Lille France
Molecular Sequence Data, Gene Dosage, Sequence Homology, Gene Expression, In Vitro Techniques, Gene, Chromosomes, B-Lymphocytes -- physiology, Promoter Regions, Genetic, DNA -- analysis, Receptors, Animals, Amino Acid Sequence, Cloning, Molecular, Promoter Regions, Genetic, Inbred F344, B cells, B-Lymphocytes, Genome, Base Sequence, Sequence Homology, Amino Acid, Interleukin -- genetics, Biologie moléculaire, Molecular, IL-5R, Chromosome Mapping, DNA, Receptors, Interleukin, 5' Untranslated Regions -- analysis, Receptors, Interleukin-5, Rats, Inbred F344, Rats, Amino Acid, Interleukin -- biosynthesis, Localization, Rat, Genetic -- genetics, Interleukin-5, 5' Untranslated Regions, Transcription, Cloning
Molecular Sequence Data, Gene Dosage, Sequence Homology, Gene Expression, In Vitro Techniques, Gene, Chromosomes, B-Lymphocytes -- physiology, Promoter Regions, Genetic, DNA -- analysis, Receptors, Animals, Amino Acid Sequence, Cloning, Molecular, Promoter Regions, Genetic, Inbred F344, B cells, B-Lymphocytes, Genome, Base Sequence, Sequence Homology, Amino Acid, Interleukin -- genetics, Biologie moléculaire, Molecular, IL-5R, Chromosome Mapping, DNA, Receptors, Interleukin, 5' Untranslated Regions -- analysis, Receptors, Interleukin-5, Rats, Inbred F344, Rats, Amino Acid, Interleukin -- biosynthesis, Localization, Rat, Genetic -- genetics, Interleukin-5, 5' Untranslated Regions, Transcription, Cloning
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