Yeast cell cycle protein CDC48p shows full-length homology to the mammalian protein VCP and is a member of a protein family involved in secretion, peroxisome formation, and gene expression.
Copyright policy )Yeast cell cycle protein CDC48p shows full-length homology to the mammalian protein VCP and is a member of a protein family involved in secretion, peroxisome formation, and gene expression.
Yeast mutants of cell cycle gene cdc48-1 arrest as large budded cells with microtubules spreading aberrantly throughout the cytoplasm from a single spindle plaque. The gene was cloned and disruption proved it to be essential. The CDC48 sequence encodes a protein of 92 kD that has an internal duplication of 200 amino acids and includes a nucleotide binding consensus sequence. Vertebrate VCP has a 70% identity over the entire length of the protein. Yeast Sec18p and mammalian N-ethylmaleimide-sensitive fusion protein, which are involved in intracellular transport, yeast Pas1p, which is essential for peroxisome assembly, and mammalian TBP-1, which influences HIV gene expression, are 40% identical in the duplicated region. Antibodies against CDC48 recognize a yeast protein of apparently 115 kD and a mammalian protein of 100 kD. Both proteins are bound loosely to components of the microsomal fraction as described for Sec18p and N-ethylmaleimide-sensitive fusion protein. This similarity suggests that CDC48p participates in a cell cycle function related to that of N-ethylmaleimide-sensitive fusion protein/Sec18p in Golgi transport.
- University of Tübingen Germany
Adenosine Triphosphatases, Saccharomyces cerevisiae Proteins, Base Sequence, Histocytochemistry, Blotting, Western, Cell Cycle, Molecular Sequence Data, Restriction Mapping, Gene Expression, Cell Cycle Proteins, Saccharomyces cerevisiae, Microbodies, Fungal Proteins, Valosin Containing Protein, Multigene Family, Mutation, Amino Acid Sequence, Cloning, Molecular, DNA, Fungal, Sequence Alignment
Adenosine Triphosphatases, Saccharomyces cerevisiae Proteins, Base Sequence, Histocytochemistry, Blotting, Western, Cell Cycle, Molecular Sequence Data, Restriction Mapping, Gene Expression, Cell Cycle Proteins, Saccharomyces cerevisiae, Microbodies, Fungal Proteins, Valosin Containing Protein, Multigene Family, Mutation, Amino Acid Sequence, Cloning, Molecular, DNA, Fungal, Sequence Alignment
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).287 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!