Identification of Novel Argonaute-Associated Proteins
Copyright policy )Identification of Novel Argonaute-Associated Proteins
RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs) . They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs . Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA . Our biochemical analysis revealed that Ago2 is present in a pre-miRNA processing complex that is able to transfer the miRNA into a target-mRNA cleaving complex. To gain insight into the function and composition of RNA silencing complexes, we purified Ago1- and Ago2-containing complexes from human cells. Several known Ago1- and/or Ago2-associated proteins including Dicer were identified, but also two novel factors, the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
Ribonuclease III, Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Eukaryotic Initiation Factor-2, Intracellular Signaling Peptides and Proteins, Oligonucleotides, Fluorescent Antibody Technique, Blotting, Northern, Mass Spectrometry, Cell Line, MicroRNAs, Peptide Initiation Factors, Argonaute Proteins, Humans, RNA-Induced Silencing Complex, RNA, Messenger, Eukaryotic Initiation Factors, RNA, Small Interfering, RNA Helicases
Ribonuclease III, Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Eukaryotic Initiation Factor-2, Intracellular Signaling Peptides and Proteins, Oligonucleotides, Fluorescent Antibody Technique, Blotting, Northern, Mass Spectrometry, Cell Line, MicroRNAs, Peptide Initiation Factors, Argonaute Proteins, Humans, RNA-Induced Silencing Complex, RNA, Messenger, Eukaryotic Initiation Factors, RNA, Small Interfering, RNA Helicases
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).483 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!