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BRG1 helps RNA polymerase II to overcome a nucleosomal barrier during elongation, in vivo

Authors: Subtil-Rodríguez, Alicia; Reyes, José C.;

BRG1 helps RNA polymerase II to overcome a nucleosomal barrier during elongation, in vivo

Abstract

RNA polymerase II (RNAPII) transcribes genes in a chromatin context. We have designed a system to investigate the role of chromatin remodelling during elongation in vivo, which involves inserting a strong nucleosome‐positioning sequence between a promoter and a reporter gene. Our data indicate that a nucleosome positioned in the body of a transcription unit impairs RNAPII progression, provokes RNAPII accumulation upstream to the positioned nucleosome and reduces transcription. By using this system, we show that BRG1, the enzymatic motor of the SWI–SNF chromatin‐remodelling complex, is recruited to the positioned nucleosome in a transcription elongation‐dependent manner and facilitates traversal of the nucleosome by RNAPII.

Related Organizations
Keywords

Genes, Reporter, DNA Helicases, Humans, Nuclear Proteins, RNA Polymerase II, Transcriptional Elongation Factors, Chromatin Assembly and Disassembly, Cell Line, Nucleosomes, Transcription Factors

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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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