ER network formation requires a balance of the dynamin-like GTPase Sey1p and the Lunapark family member Lnp1p
Copyright policy )ER network formation requires a balance of the dynamin-like GTPase Sey1p and the Lunapark family member Lnp1p
Although studies on endoplasmic reticulum (ER) structure and dynamics have focused on the ER tubule-forming proteins (reticulons and DP1/Yop1p) and the tubule fusion protein atlastin, nothing is known about the proteins and processes that act to counterbalance this machinery. Here we show that Lnp1p, a member of the conserved Lunapark family, plays a role in ER network formation. Lnp1p binds to the reticulons and Yop1p and resides at ER tubule junctions in both yeast and mammalian cells. In the yeast Saccharomyces cerevisiae, the interaction of Lnp1p with the reticulon protein, Rtn1p, and the localization of Lnp1p to ER junctions are regulated by Sey1p, the yeast orthologue of atlastin. We propose that Lnp1p and Sey1p act antagonistically to balance polygonal network formation. In support of this proposal, we show that the collapsed, densely reticulated ER network in lnp1 Δ cells is partially restored when the GTPase activity of Sey1p is abrogated.
- University of California, San Francisco United States
- University of California, San Diego United States
- UNIVERSITY OF CALIFORNIA SAN DIEGO
- Howard Hughes Medical Institute United States
- University of California, San Diego United States
Homeodomain Proteins, Binding Sites, Saccharomyces cerevisiae Proteins, Recombinant Fusion Proteins, Molecular Sequence Data, Vesicular Transport Proteins, Membrane Proteins, Membrane Transport Proteins, Saccharomyces cerevisiae, Endoplasmic Reticulum, Transfection, COS Cells, Chlorocebus aethiops, Mutation, Animals, Protein Interaction Domains and Motifs, Amino Acid Sequence, SEC Translocation Channels, Signal Transduction
Homeodomain Proteins, Binding Sites, Saccharomyces cerevisiae Proteins, Recombinant Fusion Proteins, Molecular Sequence Data, Vesicular Transport Proteins, Membrane Proteins, Membrane Transport Proteins, Saccharomyces cerevisiae, Endoplasmic Reticulum, Transfection, COS Cells, Chlorocebus aethiops, Mutation, Animals, Protein Interaction Domains and Motifs, Amino Acid Sequence, SEC Translocation Channels, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).139 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!