Early TCR αβ Expression Promotes Maturation of T Cells Expressing FcεRIγ Containing TCR/CD3 Complexes
Copyright policy )Early TCR αβ Expression Promotes Maturation of T Cells Expressing FcεRIγ Containing TCR/CD3 Complexes
AbstractIn a previous study we presented data indicating that the expanded population of CD4−CD8− (DN) αβ T cells in TCRα-chain-transgenic mice was partially if not entirely derived from γδ T cell lineage cells. The development of both γδ T cells and DN αβ T cells is poorly understood; therefore, we thought it would be important to identify the immediate precursors of the transgene-induced DN αβ T cells. We have in this report studied the early T cell development in these mice and we show that the transgenic TCRα-chain is expressed by precursor thymocytes already at the CD3−CD4−CD8− (triple negative, TN) CD44+CD25− stage of development. Both by using purified precursor populations in reconstitution experiments and by analyzing fetal thymocyte development, we demonstrated that early TN precursors expressing endogenous TCRβ-chains matured into DN αβ T cells at several stages of development. The genes encoding the γ-chain of the high affinity receptor for IgE (FcεRIγ) and the CD3ζ protein were found to be reciprocally expressed in TN thymocytes such that during development the FcεRIγ expression decreased whereas CD3ζ expression increased. Furthermore, in a fraction of the transgene-induced DN αβ T cells the FcεRIγ protein colocalized with the TCR/CD3 complex. These data suggest that similarly to γδ T cells and NKT cells, precursors expressing the TCR early in the common αβγδ developmental pathway may use the FcεRIγ protein as a signaling component of the TCR/CD3 complex.
- Lund University Sweden
Mice, Knockout, Receptors, IgE, Receptors, Antigen, T-Cell, alpha-beta, Stem Cells, Cell Differentiation, Mice, Transgenic, Thymus Gland, Immunophenotyping, Mice, Inbred C57BL, Mice, Organ Culture Techniques, Gene Expression Regulation, Receptor-CD3 Complex, Antigen, T-Cell, T-Lymphocyte Subsets, Tumor Cells, Cultured, Animals, Cell Lineage, Female, Cells, Cultured, Genes, T-Cell Receptor alpha
Mice, Knockout, Receptors, IgE, Receptors, Antigen, T-Cell, alpha-beta, Stem Cells, Cell Differentiation, Mice, Transgenic, Thymus Gland, Immunophenotyping, Mice, Inbred C57BL, Mice, Organ Culture Techniques, Gene Expression Regulation, Receptor-CD3 Complex, Antigen, T-Cell, T-Lymphocyte Subsets, Tumor Cells, Cultured, Animals, Cell Lineage, Female, Cells, Cultured, Genes, T-Cell Receptor alpha
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