This paper was concerned with the effects of ultrasonication on the gelation behavior of silk fibroins (SF), and a comparison of Domestic and Wild silkworms was studied. The results show that: with the increase of ultrasonic power, the gelation time of domestic (Bombyx Mori) SF solution decreased sharply. But wild silkworms (Antheraea yamamai and Antheraea pernyi) SF were different, When the power of utrasonication was lower than 400-500 W, the velocity of gelation were accelerated, and when the ultrasonic power was higher than 400-500W, the gelation time were delayed. Whatever domestic or wild silkworms, the mechanism of the effects of ultrasonication on the gelation behavior was that the ultrasonication promoted the structural transformation of SF molecules from random coil or α-helix to β-sheet.