AbstractAlthough an increasing number of RNA adenosine‐to‐inosine (A‐to‐I) editing sites are being discovered, how the editing frequencies of these sites are modulated to fine‐tune protein function in adaptive responses is not well understood. A previous study screening for heat tolerance in Drosophila mutants discovered a hypnos‐2 mutant strain that was later found to be defective in dADAR, the Drosophila gene encoding the A‐to‐I editing enzyme. This supports the hypothesis that cells and organisms respond to stressful environments by ADAR (adenosine deaminase acting on RNA)‐mediated RNA editing. Here, we investigated changes in the RNA A‐to‐I editing frequencies of 30 Drosophila nervous system targets in response to heat shock, a stress acclimatization that requires the dADAR function. To our surprise, most of these nervous system editing targets showed reduced editing. Our results suggest that a change in RNA editing pattern is a mechanism by which organisms acclimate to drastic environmental change. However, how RNA editing confers heat resistance is more complicated and requires further investigation.