Molecular Cloning and Post-transcriptional Regulation of Macrophage Inflammatory Protein-1α in Alveolar Macrophages
Copyright policy )Molecular Cloning and Post-transcriptional Regulation of Macrophage Inflammatory Protein-1α in Alveolar Macrophages
Macrophage inflammatory protein-1 alpha (MIP-1 alpha) belongs to the "chemokine" superfamily of chemoattractant pro-inflammatory cytokines. MIP-1 alpha is chemotactic for monocytes and neutrophils and thus, plays an important role in initiation and control of inflammation. We have isolated and sequenced a cDNA clone encoding rat MIP-1 alpha. This 0.75 kb cDNA includes a single open reading frame of 92 amino acids. Expression of MIP-1 alpha mRNA was characterized in NR8383, a rat alveolar macrophage cell line (RAM). In resting RAM cells, MIP-1 alpha mRNA decayed rapidly, with a half life of less than 2 hours. Lipopolysaccharide (LPS) treatment of RAM cells resulted in a dose-dependent increase in MIP-1 alpha steady state mRNA expression. The induction of MIP-1 alpha mRNA by LPS was partially the result of mRNA stabilization, as half life increased to over 6 hours.
- Harvard University United States
Lipopolysaccharides, Base Sequence, Monokines, Molecular Sequence Data, Macrophage Inflammatory Proteins, Cell Line, Mice, Gene Expression Regulation, Macrophages, Alveolar, Dactinomycin, Animals, Cytokines, Humans, Amino Acid Sequence, Cloning, Molecular, Chemokine CCL4, Cells, Cultured, DNA Primers, Gene Library, Half-Life
Lipopolysaccharides, Base Sequence, Monokines, Molecular Sequence Data, Macrophage Inflammatory Proteins, Cell Line, Mice, Gene Expression Regulation, Macrophages, Alveolar, Dactinomycin, Animals, Cytokines, Humans, Amino Acid Sequence, Cloning, Molecular, Chemokine CCL4, Cells, Cultured, DNA Primers, Gene Library, Half-Life
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