RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
Copyright policy )RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
Dnmt2 proteins are the most conserved members of the DNA methyltransferase enzyme family, but their substrate specificity and biological functions have been a subject of controversy. We show here that, in addition to tRNAAsp-GTC, tRNAVal-AAC and tRNAGly-GCC are also methylated by Dnmt2. Drosophila Dnmt2 mutants showed reduced viability under stress conditions, and Dnmt2 relocalized to stress granules following heat shock. Strikingly, stress-induced cleavage of tRNAs was Dnmt2-dependent, and Dnmt2-mediated methylation protected tRNAs against ribonuclease cleavage. These results uncover a novel biological function of Dnmt2-mediated tRNA methylation, and suggest a role for Dnmt2 enzymes during the biogenesis of tRNA-derived small RNAs.
- National Research Council Italy
- Johannes Gutenberg University of Mainz Germany
- German Cancer Research Center Germany
- Institute of Genetics and Biophysics Italy
- Heidelberg University Germany
Male, Ribonuclease, Pancreatic, Methylation, Survival Analysis, Mice, Drosophila melanogaster, RNA, Transfer, Stress, Physiological, Animals, Drosophila Proteins, RNA, Female, DNA (Cytosine-5-)-Methyltransferases, Sequence Deletion
Male, Ribonuclease, Pancreatic, Methylation, Survival Analysis, Mice, Drosophila melanogaster, RNA, Transfer, Stress, Physiological, Animals, Drosophila Proteins, RNA, Female, DNA (Cytosine-5-)-Methyltransferases, Sequence Deletion
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