Objectives: To evaluate the impact of epinephrine, norepinephrine, or placebo on the ventricular fibrillation cycle length (VFCL) and the variability of VFCL (cvVFCL) measurements in implantable cardioverter defibrillator (ICD) patients with or without beta‐blockers. Methods: Forty‐three patients scheduled for their 6‐week post‐ICD placement noninvasive electrophysiologic study were included in the study at the Arrhythmia Procedure Laboratory at Hartford Hospital, Hartford, CT. This randomized, double‐blind, placebo‐controlled evaluation was approved by the Hartford Hospital Institutional Review Board. Results: After 2 seconds of continuous VF, 7 consecutive VFCLs were measured from the ICD device recording printout using a 0.5 mm scale ruler under magnification at baseline and after the infusion of catecholamines (epinephrine or norepinephrine at 2 mcg/min) or matching placebo at steady state. The average VFCL and the cvVFCL were determined for each study phase. Subgroup analysis based on chronic beta‐blocker use was performed. No between‐group differences were noted for epinephrine, norepinephrine, or placebo group for baseline (P = 0.538) or postinfusion VFCL (P = 0.749) or for baseline (P = 0.561) or postinfusion cvVFCL (P = 0.623) Regardless of catecholamine group randomization, longer pre‐ and postinfusion VFCL were noted in those receiving beta‐blockers (P = 0.157, P = 0.019) but no differences in cvVFCL were noted (P = 0.216, P = 0.474) versus those without beta‐blockers, respectively. Conclusion: Moderately dosed epinephrine or norepinephrine does not affect either VFCL or the variability of VFCL after short duration of ventricular fibrillation. Chronic cardioselective beta‐blockade prolongs VFCL without any impact on coefficient of variation of VFCL.