Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere.
Copyright policy )Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere.
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. To examine the molecular basis for the specification of centromeric chromatin, we have cloned a human cDNA that encodes the 17-kD histone-like centromere antigen, CENP-A. Two domains are evident in the 140 aa CENP-A polypeptide: a unique NH2-terminal domain and a 93-amino acid COOH-terminal domain that shares 62% identity with nucleosomal core protein, histone H3. An epitope tagged derivative of CENP-A was faithfully targeted to centromeres when expressed in a variety of animal cells and this targeting activity was shown to reside in the histone-like COOH-terminal domain of CENP-A. These data clearly indicate that the assembly of centromeres is driven, at least in part, by the incorporation of a novel core histone into centromeric chromatin.
- Scripps Research Institute United States
Protein Folding, Base Sequence, Sequence Homology, Amino Acid, Chromosomal Proteins, Non-Histone, Recombinant Fusion Proteins, Centromere, Immunoblotting, Molecular Sequence Data, Fluorescent Antibody Technique, Autoantigens, Histones, Muntjacs, Oligodeoxyribonucleotides, Animals, Humans, Cattle, Amino Acid Sequence, Cloning, Molecular, Centromere Protein A, HeLa Cells
Protein Folding, Base Sequence, Sequence Homology, Amino Acid, Chromosomal Proteins, Non-Histone, Recombinant Fusion Proteins, Centromere, Immunoblotting, Molecular Sequence Data, Fluorescent Antibody Technique, Autoantigens, Histones, Muntjacs, Oligodeoxyribonucleotides, Animals, Humans, Cattle, Amino Acid Sequence, Cloning, Molecular, Centromere Protein A, HeLa Cells
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