αCP Poly(C) Binding Proteins Act as Global Regulators of Alternative Polyadenylation
Copyright policy )αCP Poly(C) Binding Proteins Act as Global Regulators of Alternative Polyadenylation
We have previously demonstrated that the KH-domain protein αCP binds to a 3' untranslated region (3'UTR) C-rich motif of the nascent human alpha-globin (hα-globin) transcript and enhances the efficiency of 3' processing. Here we assess the genome-wide impact of αCP RNA-protein (RNP) complexes on 3' processing with a specific focus on its role in alternative polyadenylation (APA) site utilization. The major isoforms of αCP were acutely depleted from a human hematopoietic cell line, and the impact on mRNA representation and poly(A) site utilization was determined by direct RNA sequencing (DRS). Bioinformatic analysis revealed 357 significant alterations in poly(A) site utilization that could be specifically linked to the αCP depletion. These APA events correlated strongly with the presence of C-rich sequences in close proximity to the impacted poly(A) addition sites. The most significant linkage was the presence of a C-rich motif within a window 30 to 40 bases 5' to poly(A) signals (AAUAAA) that were repressed upon αCP depletion. This linkage is consistent with a general role for αCPs as enhancers of 3' processing. These findings predict a role for αCPs in posttranscriptional control pathways that can alter the coding potential and/or levels of expression of subsets of mRNAs in the mammalian transcriptome.
- University of California, Los Angeles United States
- University of Pennsylvania United States
- University of Chicago United States
- University of Iowa United States
Binding Sites, Base Sequence, Molecular Sequence Data, RNA-Binding Proteins, Polyadenylation, Heterogeneous-Nuclear Ribonucleoproteins, DNA-Binding Proteins, alpha-Globins, Humans, RNA, Messenger, RNA 3' End Processing, K562 Cells, Poly A, Transcriptome, 3' Untranslated Regions
Binding Sites, Base Sequence, Molecular Sequence Data, RNA-Binding Proteins, Polyadenylation, Heterogeneous-Nuclear Ribonucleoproteins, DNA-Binding Proteins, alpha-Globins, Humans, RNA, Messenger, RNA 3' End Processing, K562 Cells, Poly A, Transcriptome, 3' Untranslated Regions
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).40 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!