Development and characterization of a novel human Waldenström macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute – Waldenström Macroglobulinemia 1
Copyright policy )Development and characterization of a novel human Waldenström macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute – Waldenström Macroglobulinemia 1
Understanding the biology of Waldenström macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal chromosomal number of 46 and harbors chromosomal changes such as deletion of 6q21, monoallelic deletion of 9p21 (CDKN2A), 13q14 (RB1) and 18q21 (BCL-2), with a consistent amplification of 14q32 (immunoglobulin heavy chain; IgH) identical to its founding tumor sample. The clonal relationship is confirmed by identical CDR3 length and single nucleotide polymorphisms as well as a matching IgH sequence of the cell line and founding tumor. Both also harbor a heterozygous, non-synonymous mutation at amino acid 265 in the MYD88 gene (L265P). The cell line expresses most of the cell surface markers present on the parent cells. Overall, RPCI-WM1 represents a valuable model to study Waldenström macroglobulinemia.
- University of California System United States
- State University of New York at Potsdam United States
- Mayo Clinic United States
- Icahn School of Medicine at Mount Sinai United States
- University of Southern California United States
Base Sequence, Molecular Sequence Data, Transplantation, Heterologous, Middle Aged, Polymorphism, Single Nucleotide, Immunophenotyping, Disease Models, Animal, Immunoglobulin kappa-Chains, Mice, Immunoglobulin M, Cell Line, Tumor, Cytogenetic Analysis, Mutation, Myeloid Differentiation Factor 88, Animals, Humans, Female, Waldenstrom Macroglobulinemia, Immunoglobulin Heavy Chains, Sequence Alignment
Base Sequence, Molecular Sequence Data, Transplantation, Heterologous, Middle Aged, Polymorphism, Single Nucleotide, Immunophenotyping, Disease Models, Animal, Immunoglobulin kappa-Chains, Mice, Immunoglobulin M, Cell Line, Tumor, Cytogenetic Analysis, Mutation, Myeloid Differentiation Factor 88, Animals, Humans, Female, Waldenstrom Macroglobulinemia, Immunoglobulin Heavy Chains, Sequence Alignment
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