Homeobox proteins are transcription factors known to be involved in the molecular basics of body model formation and transformation. Some homeobox proteins are known to play critical roles in the control of the extraembryonic development of the placenta during gestation and in the regulation of uterine and placental physiology in adults. The gravid uteruses and placentas of 27 Holsteins cows, obtained from private slaughterhouses, were used in this study. The tissues were assigned to three groups as belonging to the first, second and third timesters of gestation, based on the fetal ages determined. Subsequently, the tissues were subjected to immunohistochemical staining using antibodies specific to the proteins investigated in the study. The evaluation of the immunohistochemical findings obtained in this study, demonstrated the presence of trimester-dependent varying intensities of immunoreactions in the uterus and placenta. Immunoreactivity was observed particularly in the luminal and glandular epithelial cells of the uterus, as well as in stromal and some endothelial cells. Furthermore, immunoreactivity for the proteins HOXA10, HOXB6, HOXC6 and Dlx-5 was determined in the smooth muscle cells. Moreover, immunoreactivity was also detected in the maternal epithelium and fetal trophoblasts found in the structure of the placenta. The results suggest that the homeobox proteins investigated may have critical roles in the regulation of endometrial functions in cows, and the proliferation and differentiation of endometrial and placental cells. It is concluded that these proteins may have physiological roles in the formation and development of the placenta, as well as in the maintenance of pregnancy.