DGKζ is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway
Copyright policy )DGKζ is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway
Diacylglycerol kinase (DGK) plays an important role in phosphoinositide signaling cascade by regulating the intracellular level of diacylglycerol and phosphatidic acid. The DGK family is involved in various pathophysiological responses that are mediated through unique binding partners in different tissues and cells. In this study, we identified a small GTPase effector protein, IQGAP1, as a novel DGKζ-associated complex protein. A bacterial endotoxin, lipopolysaccharide (LPS), facilitated the complex formation in macrophages. Both proteins co-localized at the edge and phagocytic cup of the cell. Furthermore, RNA interference-mediated knockdown of DGKζ or IQGAP1 impaired LPS-induced Rac1 activation. Primary macrophages derived from DGKζ(-/-) mice attenuated LPS-induced phagocytosis of bacteria. These results suggest that DGKζ is involved in IQGAP1/Rac1-mediated phagocytosis upon LPS stimulation in macrophages.
- Juntendo University Japan
- University of Utah United States
- Nagoya University Japan
- University of Hyogo Japan
- Huntsman Cancer Institute United States
Lipopolysaccharides, rac1 GTP-Binding Protein, Diacylglycerol Kinase, Macrophages, Neuropeptides, Macrophage Activation, rac GTP-Binding Proteins, Mice, Inbred C57BL, Mice, Phagocytosis, ras GTPase-Activating Proteins, Animals, Humans, Cells, Cultured
Lipopolysaccharides, rac1 GTP-Binding Protein, Diacylglycerol Kinase, Macrophages, Neuropeptides, Macrophage Activation, rac GTP-Binding Proteins, Mice, Inbred C57BL, Mice, Phagocytosis, ras GTPase-Activating Proteins, Animals, Humans, Cells, Cultured
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