Identification of Δ5-Desaturase from Mortierella alpina by Heterologous Expression in Bakers' Yeast and Canola
Copyright policy )Identification of Δ5-Desaturase from Mortierella alpina by Heterologous Expression in Bakers' Yeast and Canola
A DNA fragment with homology to Delta6-desaturases from borage and cyanobacteria was isolated after polymerase chain reaction amplification of Mortierella alpina cDNA with oligonucleotide primers corresponding to the conserved regions of known Delta6-desaturase genes. This fragment was used as a probe to isolate a cDNA clone with an open reading frame encoding 446 amino acids from a M. alpina library. Expression of this open reading frame from an inducible promoter in Saccharomyces cerevisiae in the presence of various substrates revealed that the recombinant product had Delta5-desaturase activity. The effects of growth and induction conditions as well as host strain on activity of the recombinant Delta5-desaturase in S. cerevisiae were evaluated. Expression of the M. alpina Delta5-desaturase cDNA in transgenic canola seeds resulted in the production of taxoleic acid (Delta5,9-18:2) and pinolenic acid (Delta5,9,12-18:3), which are the Delta5-desaturation products of oleic and linoleic acids, respectively.
- Abbott (Germany) Germany
Fatty Acid Desaturases, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Saccharomyces cerevisiae, Plants, Genetically Modified, Gas Chromatography-Mass Spectrometry, Recombinant Proteins, Mortierella, Seeds, Amino Acid Sequence, Cloning, Molecular, DNA Primers
Fatty Acid Desaturases, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Saccharomyces cerevisiae, Plants, Genetically Modified, Gas Chromatography-Mass Spectrometry, Recombinant Proteins, Mortierella, Seeds, Amino Acid Sequence, Cloning, Molecular, DNA Primers
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).145 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!