Hypoxia increases cytoplasmic expression of NDRG1, but is insufficient for its membrane localization in human hepatocellular carcinoma
Copyright policy )Hypoxia increases cytoplasmic expression of NDRG1, but is insufficient for its membrane localization in human hepatocellular carcinoma
NDRG1 is a hypoxia‐inducible protein, whose modulated expression is associated with the progression of human cancers. Here, we reveal that NDRG1 is markedly upregulated in the cytoplasm and on the membrane in human hepatocellular carcinoma (HCC). We demonstrate further that hypoxic stress increases the cytoplasmic expression of NDRG1 in vitro, but does not result in its localization on the plasma membrane. However, grown within an HCC‐xenograft in vivo, cells express NDRG1 in the cytoplasm and on the plasma membrane. In conclusion, hypoxia is a potent inducer of NDRG1 in HCCs, albeit requiring additional stimuli within the tumour microenvironment for its recruitment to the membrane.
- University of Bern Switzerland
- University of Bern Switzerland
Male, Cytoplasm, Carcinoma, Hepatocellular, Molecular Sequence Data, Gene Expression, Mice, Nude, Cell Cycle Proteins, In Vitro Techniques, Mice, Cell Line, Tumor, Animals, Humans, Amino Acid Sequence, Hypoxia, Carcinoma, hepatocellular, Cell Membrane, Liver Neoplasms, HIF-1, Intracellular Signaling Peptides and Proteins, Hypoxia-Inducible Factor 1, alpha Subunit, N-myc downstream-regulated gene 1, Protein Structure, Tertiary, Neoplasm Transplantation
Male, Cytoplasm, Carcinoma, Hepatocellular, Molecular Sequence Data, Gene Expression, Mice, Nude, Cell Cycle Proteins, In Vitro Techniques, Mice, Cell Line, Tumor, Animals, Humans, Amino Acid Sequence, Hypoxia, Carcinoma, hepatocellular, Cell Membrane, Liver Neoplasms, HIF-1, Intracellular Signaling Peptides and Proteins, Hypoxia-Inducible Factor 1, alpha Subunit, N-myc downstream-regulated gene 1, Protein Structure, Tertiary, Neoplasm Transplantation
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