Additional file 2: Supplementary Figure 2. Sox10 induction is preferentially mediated by AKT2. (A) Chromatin immunoprecipitation (ChIP) was performed on SLKfl/fl and SLK-/- NDL cells to assess K27 Acetylated histone H3 binding to the Sox10 enhancers. Following ChIP, qPCR analysis was performed across two putative SoxE binding sites within the -6904/-5995 fragment of the Sox10 promoter. qRT-PCR data was normalized to an IgG ChIP or a negative control element within exon one (-150/+103) as in Fig. 2. No statistical differences were observed between the cell lines. N=3. (B) Knock down of AKT1 or AKT2 results in Sox10 downregulation. AKT1 knockdowns were ~50% at best with two independent siRNAs. AKT2 knockdowns with two siRNAs ranged from 50-70% with a marked downregulation (80-90%) of Sox10. (C) AKT3 knockdowns from 40-60% did not show any appreciable reduction in Sox10 levels. Underexposed blots were subjected to ImageJ densitometry and normalized to β-actin and NT controls. NT= non targeting siRNA control.