Heparanase Expression and TrkC/p75NTR Ratios in Human Medulloblastoma
Copyright policy )Heparanase Expression and TrkC/p75NTR Ratios in Human Medulloblastoma
Medulloblastoma (MB), the most devastating and common brain tumor in children, is highly invasive and extremely difficult to treat. Identifying the properties of MB tumors that cause them to invade and metastasize is therefore imperative for the development of novel treatments. We performed investigations to elucidate prognostic implications of heparanase (HPSE-1) and TrkC/p75(NTR) expression in MB using formalin-fixed, paraffin-embedded (FFPE) MB clinical specimens from children aged 1-19 years. Expressions of p75(NTR) and HPSE-1 correlated with each other (Pearson's correlation R = 0.899; P < 0.0001; R (2) = 81%; n = 14). In addition, TrkC:p75(NTR) ratios correlated with MB meningeal spread (R = 0.608; P = 0.0212; R (2) = 37%; n = 14). Secondly, using antibodies specific to TrkC and HPSE-1, we carried out immunohistochemistry (IHC) on 22 human MB tissue samples. IHC reaction scores revealed a significant expression of HPSE-1 in 76% of MB tissues from children aged 3 years and older (P = 0.0490; n = 17) while TrkC immunoreactivity was detected in 71% of these patient samples. Of note, TrkC was significantly present in 100% of MB female patients (P = 0.0313; n = 6). These studies support the role of p75(NTR) and HPSE-1 as two novel molecular determinants involved in the biology and clinical progression of MB.
- Louisiana State University United States
- University of Arkansas for Medical Sciences United States
- The University of Texas MD Anderson Cancer Center United States
Male, DNA, Complementary, Nerve Tissue Proteins, Receptors, Nerve Growth Factor, Immunohistochemistry, Polymerase Chain Reaction, Child, Preschool, Humans, Female, Receptor, trkC, RNA, Neoplasm, Cerebellar Neoplasms, Child, DNA Primers, Glucuronidase, Medulloblastoma
Male, DNA, Complementary, Nerve Tissue Proteins, Receptors, Nerve Growth Factor, Immunohistochemistry, Polymerase Chain Reaction, Child, Preschool, Humans, Female, Receptor, trkC, RNA, Neoplasm, Cerebellar Neoplasms, Child, DNA Primers, Glucuronidase, Medulloblastoma
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!