A Rsc3/Rsc30 Zinc Cluster Dimer Reveals Novel Roles for the Chromatin Remodeler RSC in Gene Expression and Cell Cycle Control
Copyright policy )A Rsc3/Rsc30 Zinc Cluster Dimer Reveals Novel Roles for the Chromatin Remodeler RSC in Gene Expression and Cell Cycle Control
Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts- mutants display a G2/M cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30Delta mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable Rsc3/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC.
- Huntsman Cancer Institute United States
- University of Utah United States
- Memorial Sloan Kettering Cancer Center United States
- University of Utah Health Care United States
- Howard Hughes Medical Institute United States
G2 Phase, Ribosomal Proteins, Leucine Zippers, Saccharomyces cerevisiae Proteins, Base Sequence, Cell Cycle, Molecular Sequence Data, Mitosis, Nuclear Proteins, Cell Biology, Spindle Apparatus, Chromatin, Protein Structure, Secondary, Protein Structure, Tertiary, DNA-Binding Proteins, Genes, cdc, Cell Wall, Gene Expression Regulation, Fungal, Mutagenesis, Site-Directed, Genes, Lethal, Molecular Biology, Dimerization
G2 Phase, Ribosomal Proteins, Leucine Zippers, Saccharomyces cerevisiae Proteins, Base Sequence, Cell Cycle, Molecular Sequence Data, Mitosis, Nuclear Proteins, Cell Biology, Spindle Apparatus, Chromatin, Protein Structure, Secondary, Protein Structure, Tertiary, DNA-Binding Proteins, Genes, cdc, Cell Wall, Gene Expression Regulation, Fungal, Mutagenesis, Site-Directed, Genes, Lethal, Molecular Biology, Dimerization
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