The Arg/N-degron pathway targets transcription factors and regulates specific genes
Copyright policy )The Arg/N-degron pathway targets transcription factors and regulates specific genes
Significance The Arg/N-degron pathway targets proteins for degradation by recognizing their N-terminal or internal degradation signals. In the present study, we compared wild-type human cells to their double-knockout (2-KO) counterparts that lacked the UBR1/UBR2 ubiquitin ligases of the Arg/N-degron pathway. We found that a number of specific genes were either strongly induced or strongly repressed in 2-KO cells. In addition, specific transcription factors, including glucocorticoid receptor, were identified here as physiological substrates of the Arg/N-degron pathway. We discuss the emerged role of this proteolytic system as a regulator of mammalian gene expression.
- California Institute of Technology United States
- Harvard University United States
PREP1, Monocarboxylic Acid Transporters, 570, Intermediate Filaments, UBR1, Mass Spectrometry, Gene Knockout Techniques, Receptors, Glucocorticoid, Antigens, Neoplasm, Humans, RNA-Seq, Eye Proteins, degradation, Homeodomain Proteins, Activating Transcription Factor 3, Microfilament Proteins, Neoplasm Proteins, GR, HEK293 Cells, Gene Expression Regulation, Proteolysis, Receptors, Adrenergic, beta-2, transcription, Protein Binding, Signal Transduction, Transcription Factors
PREP1, Monocarboxylic Acid Transporters, 570, Intermediate Filaments, UBR1, Mass Spectrometry, Gene Knockout Techniques, Receptors, Glucocorticoid, Antigens, Neoplasm, Humans, RNA-Seq, Eye Proteins, degradation, Homeodomain Proteins, Activating Transcription Factor 3, Microfilament Proteins, Neoplasm Proteins, GR, HEK293 Cells, Gene Expression Regulation, Proteolysis, Receptors, Adrenergic, beta-2, transcription, Protein Binding, Signal Transduction, Transcription Factors
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