Differential gene expression identified in Uigur women cervical squamous cell carcinoma by suppression subtractive hybridization
Copyright policy )Differential gene expression identified in Uigur women cervical squamous cell carcinoma by suppression subtractive hybridization
Cervical cancer is one of the most common gynecological cancers worldwide. Over the past decade, much progress has been made in understanding the genetic changes associated with the development and progression of cervical cancer. However, the precise mechanisms of cervical carcinogenesis in Uigur women remain unclear. To screen differential gene expression in squamous cell carcinoma (SCC) of the cervix in Uigur women, suppressive subtractive hybridization (SSH) was performed on the cervical squamous cell carcinoma and corresponding normal cervical tissues of a Uigur patient. Thus we were be able to find the genes that are related with cervical tumors of Uigur women. A total of 300 samples were subject to DNA sequencing analysis and 46 genes were found to express differentially in tumors compared with normal tissues. Of the 46 genes, 24 genes were up-regulated whereas 22 genes were down-regulated in cervical tumors. The expression profiles of 5 of the 46 genes were further confirmed in 15 other Uigur patients by semi-quantitative reverse-transcription polymerase chain reaction. Our results revealed that ACADVL, CEBPB, IFITM1 and DNAJC9 are involved in cervical carcinogenesis.
- University of the Sunshine Coast Australia
- Shihezi University China (People's Republic of)
- University of Queensland Australia
- University of Queensland Australia
- Huazhong University of Science and Technology China (People's Republic of)
squamous cell carcinoma, Adult, China, Uterine Cervical Neoplasms, cervical tumor, suppression subtractive hybridization, 616, Biomarkers, Tumor, Humans, 1306 Cancer Research, RNA, Messenger, Uigur women, Neoplasm Staging, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, subtractive hybridization, Nucleic Acid Hybridization, Middle Aged, Prognosis, Uigur women squamous cell carcinoma, Gene Expression Regulation, Neoplastic, FoR 1112 (Oncology and Carcinogenesis), gene expression, Carcinoma, Squamous Cell, Female, supression
squamous cell carcinoma, Adult, China, Uterine Cervical Neoplasms, cervical tumor, suppression subtractive hybridization, 616, Biomarkers, Tumor, Humans, 1306 Cancer Research, RNA, Messenger, Uigur women, Neoplasm Staging, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, subtractive hybridization, Nucleic Acid Hybridization, Middle Aged, Prognosis, Uigur women squamous cell carcinoma, Gene Expression Regulation, Neoplastic, FoR 1112 (Oncology and Carcinogenesis), gene expression, Carcinoma, Squamous Cell, Female, supression
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