The actin‐binding protein Filamin‐A interacts with the metabotropic glutamate receptor type 7
Copyright policy )The actin‐binding protein Filamin‐A interacts with the metabotropic glutamate receptor type 7
A yeast two‐hybrid screen identified Filamin‐A as a binding partner of the metabotropic glutamate receptor type 7b (mGluR7b) splice variant. In addition, Filamin‐A interacted with mGluR4a, mGluR5a, mGluR5b, mGluR7a and mGluR8a. Domain mapping revealed that alternative splicing of mGluR4, mGluR7 and mGluR8 C‐termini regulated the interaction. A conserved tyrosine within mGluR C‐termini was identified to mediate the binding to Filamin‐A. Protein interactions were verified in biochemical assays using recombinant and native proteins. Finally, co‐expression of Filamin‐A and mGluR7 splice variants was shown in brain regions. These findings suggest that Filamin‐A may physically link metabotropic glutamate receptors to the actin cytoskeleton.
- University of Erlangen-Nuremberg Germany
Filamins, Receptors, Metabotropic Glutamate, Cell Line, Mice, Contractile Proteins, Two-Hybrid System Techniques, mGluR, Filamin-A, Animals, Humans, Protein Isoforms, Expression pattern, Microfilament Proteins, Yeast two-hybrid, Brain, Recombinant Proteins, Protein Structure, Tertiary, Rats, Protein–protein interaction, Alternative Splicing, Organ Specificity, Cattle, Protein Binding
Filamins, Receptors, Metabotropic Glutamate, Cell Line, Mice, Contractile Proteins, Two-Hybrid System Techniques, mGluR, Filamin-A, Animals, Humans, Protein Isoforms, Expression pattern, Microfilament Proteins, Yeast two-hybrid, Brain, Recombinant Proteins, Protein Structure, Tertiary, Rats, Protein–protein interaction, Alternative Splicing, Organ Specificity, Cattle, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).60 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!