CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1α/CXCR4 signaling axis
Copyright policy )CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1α/CXCR4 signaling axis
Bone mesenchymal stem cells (BMSCs) are attractive candidates for cell based therapies to cardiovascular disease such as infarction and atherosclerosis; however, the mechanisms responsible for stem cell chemotaxis and homing remain unknown. Chemokine stromal cell-derived factor 1 (SDF-1alpha) is involved in the process of atherogenesis. This study was aimed at investigating whether the SDF-1alpha of human umbilical vein endothelial cells (HUVECs) plays a role in migration of BM-derived CXCR4(+)(receptor for SDF-1alpha) stem cells.HUVECs were cultured from human umbilical cords and was treated with ox-LDL. The mRNA and protein expression of SDF-1alpha was detected in HUVECs. CXCR4(+)BMSCs from bone marrow were isolated and were tested by migration and adhesion assays.It was found that ox-LDL induced HUVECs to increase the mRNA and protein expression of SDF-1alpha. Ox-LDL increased the migratory and adhesion response of CXCR4(+)BMSCs. When the neutralizing SDF-1alpha antibody abrogated the secreted SDF-1alpha, the migration and adhesion response of CXCR4(+)BMSCs markedly decreased.Our data indicated that the endothelial cells (ECs) stimulated by ox-LDL could increase the BMSCs migratory response via SDF-1alpha/CXCR4 signaling axis. These findings provide a new paradigm for biological effects of ox-LDL and have implications for novel stem cell therapeutic strategies for atherosclerosis.
- Huazhong University of Science and Technology China (People's Republic of)
- Tongji Hospital China (People's Republic of)
Receptors, CXCR4, Umbilical Veins, Reverse Transcriptase Polymerase Chain Reaction, Chemotaxis, Cell Culture Techniques, Enzyme-Linked Immunosorbent Assay, Mesenchymal Stem Cells, Thiobarbituric Acid Reactive Substances, Bone and Bones, Chemokine CXCL12, Recombinant Proteins, Lipoproteins, LDL, Cell Movement, Humans, RNA, Endothelium, Vascular, RNA, Messenger, DNA Primers, Signal Transduction
Receptors, CXCR4, Umbilical Veins, Reverse Transcriptase Polymerase Chain Reaction, Chemotaxis, Cell Culture Techniques, Enzyme-Linked Immunosorbent Assay, Mesenchymal Stem Cells, Thiobarbituric Acid Reactive Substances, Bone and Bones, Chemokine CXCL12, Recombinant Proteins, Lipoproteins, LDL, Cell Movement, Humans, RNA, Endothelium, Vascular, RNA, Messenger, DNA Primers, Signal Transduction
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