The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export
Copyright policy )The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export
Many cellular responses rely on the control of nucleocytoplasmic transport of transcriptional regulators. The Drosophila nucleoporin Nup88 is selectively required for nuclear accumulation of Rel proteins and full activation of the innate immune response. Here, we investigate the mechanisms underlying its role in nucleocytoplasmic transport. Nuclear import of an nuclear localization signal-enhanced green fluorescent protein (NLS-EGFP) reporter is not affected in DNup88 (members only; mbo) mutants, whereas the level of CRM1-dependent EGFP-nuclear export signal (EGFP-NES) export is increased. We show that the nuclear accumulation of the Drosophila Rel protein Dorsal requires CRM1. DNup88 binds to DNup214 and DCRM1 in vitro, and both proteins become mislocalized from the nuclear rim into the nucleus of mbo mutants. Overexpression of DNup88 is sufficient to relocalize DNup214 and CRM1 on the nuclear envelope and revert the mutant phenotypes. We propose that a major function of DNup88 is to anchor DNup214 and CRM1 on the nuclear envelope and thereby attenuate NES-mediated nuclear export.
- Stockholm University Sweden
- University of Gothenburg Sweden
- Antoni van Leeuwenhoek Hospital Netherlands
- Netherlands Heart Institute Netherlands
Nuclear Envelope, Recombinant Fusion Proteins, Nuclear Localization Signals, Active Transport, Cell Nucleus, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, Exportin 1 Protein, Karyopherins, Phosphoproteins, Nuclear Pore Complex Proteins, Report, Mutation, Animals, Drosophila Proteins, Drosophila, Transcription Factors
Nuclear Envelope, Recombinant Fusion Proteins, Nuclear Localization Signals, Active Transport, Cell Nucleus, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, Exportin 1 Protein, Karyopherins, Phosphoproteins, Nuclear Pore Complex Proteins, Report, Mutation, Animals, Drosophila Proteins, Drosophila, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).78 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!