Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation
Copyright policy )Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation
Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-γ secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-γ, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice.
- University of Hong Kong China (People's Republic of)
- Jiangsu University China (People's Republic of)
- University of Hong Kong (香港大學) China (People's Republic of)
- Centers for Disease Control and Prevention United States
Homeodomain Proteins, Antigen Presentation, Cell Differentiation, Dendritic Cells, Interleukin-12, Cell Line, Interferon-gamma, Mice, Gene Expression Regulation, Animals, Biomarkers, Transcription Factors
Homeodomain Proteins, Antigen Presentation, Cell Differentiation, Dendritic Cells, Interleukin-12, Cell Line, Interferon-gamma, Mice, Gene Expression Regulation, Animals, Biomarkers, Transcription Factors
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