Critical Contribution of Linker Proteins to Raf Kinase Activation
Copyright policy )Critical Contribution of Linker Proteins to Raf Kinase Activation
Genetic analysis of Ras signaling has unveiled the participation of non-enzymatic accessory proteins in signal transmission. These proteins, KSR, CNK, and Sur-8, can interact with multiple core components of the Ras/MAP kinase cascade and may contribute to the structural organization of this cascade. However, the precise biochemical nature of the contribution of these proteins to Ras signaling is currently unknown. Here we show directly that CNK and KSR are required for stimulus dependent Raf kinase activation. CNK is required for membrane recruitment of Raf, while KSR is likely required to couple Raf to upstream kinases. These results demonstrate that CNK and KSR are integral components of the cellular machinery mediating Raf activation.
- The University of Texas Southwestern Medical Center United States
- The University of Texas at Dallas United States
- The University of Texas System United States
Culture Media, Serum-Free, Cell Line, Enzyme Activation, Proto-Oncogene Proteins c-raf, Genes, ras, Gene Expression Regulation, Animals, Drosophila Proteins, Insect Proteins, Drosophila, Mitogen-Activated Protein Kinases, Caenorhabditis elegans Proteins, Carrier Proteins, Protein Kinases, Adaptor Proteins, Signal Transducing, RNA, Double-Stranded, Signal Transduction
Culture Media, Serum-Free, Cell Line, Enzyme Activation, Proto-Oncogene Proteins c-raf, Genes, ras, Gene Expression Regulation, Animals, Drosophila Proteins, Insect Proteins, Drosophila, Mitogen-Activated Protein Kinases, Caenorhabditis elegans Proteins, Carrier Proteins, Protein Kinases, Adaptor Proteins, Signal Transducing, RNA, Double-Stranded, Signal Transduction
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