Effects of corticosterone deficiency and its replacement on leydig cell steroidogenesis
Copyright policy )Effects of corticosterone deficiency and its replacement on leydig cell steroidogenesis
AbstractClinical and experimental studies have shown the adverse effects of glucocorticoid deficiency/metyrapone treatment on testicular Leydig cell testosterone production. However, molecular mechanisms that underlie the effects of glucocorticoid deficiency on Leydig cell steroidogenesis are not yet determined. Therefore, the present study was designed to assess the mechanism of this phenomenon. Following metyrapone‐induced corticosterone deficiency, serum testosterone, and Leydig cell 14C‐glucose oxidation were decreased. StAR mRNA and protein levels were significantly increased in Leydig cells of corticosterone‐deficient animals. mRNA levels and the specific activities of P450scc and 17β‐HSD were decreased by corticosterone deficiency, whereas the activity and mRNA of 3β‐HSD were increased. Simultaneous administration of corticosterone prevented its deficiency‐induced changes in Leydig cells. Our results show that metyrapone‐induced corticosterone deficiency impairs Leydig cell testosterone production by decreasing the activities of steroidogenic enzymes and their mRNA expression and glucose oxidation. J. Cell. Biochem. 104: 1671–1683, 2008. © 2008 Wiley‐Liss, Inc.
- University of Madras India
Male, 3-Hydroxysteroid Dehydrogenases, 17-Hydroxysteroid Dehydrogenases, Hormone Replacement Therapy, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Leydig Cells, Metyrapone, Phosphoproteins, Rats, Glucose, Gene Expression Regulation, Animals, Testosterone, Carbon Radioisotopes, Cholesterol Side-Chain Cleavage Enzyme, RNA, Messenger, Rats, Wistar, Corticosterone, Oxidation-Reduction
Male, 3-Hydroxysteroid Dehydrogenases, 17-Hydroxysteroid Dehydrogenases, Hormone Replacement Therapy, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Leydig Cells, Metyrapone, Phosphoproteins, Rats, Glucose, Gene Expression Regulation, Animals, Testosterone, Carbon Radioisotopes, Cholesterol Side-Chain Cleavage Enzyme, RNA, Messenger, Rats, Wistar, Corticosterone, Oxidation-Reduction
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