In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing α-Mannosidase-Like Proteins
In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing α-Mannosidase-Like Proteins
Quality control of newly synthesized glycoproteins is tightly regulated by sugar processing of N-glycans and by recognition of specific glycan structures by lectins in the endoplasmic reticulum (ER). Mannose trimming and its recognition determine the targeting of misfolded glycoproteins for ER-associated degradation. ER degradation-enhancing α-mannosidase-like (EDEM) proteins in mammals and their homologue Htm1p/Mnl1p in Saccharomyces cerevisiae are involved in this process. To analyze the function of EDEM proteins, we expressed and purified recombinant EDEM3 from HEK293 cells and assessed its mannose-trimming activity in vitro.
- Kyoto University Japan
Quality Control, Protein Folding, HEK293 Cells, alpha-Mannosidase, Calcium-Binding Proteins, Humans, Endoplasmic Reticulum, Mannose
Quality Control, Protein Folding, HEK293 Cells, alpha-Mannosidase, Calcium-Binding Proteins, Humans, Endoplasmic Reticulum, Mannose
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