Subcellular Localization and Differentiation-Induced Redistribution of the Protein Tyrosine Phosphatase PTP-BL in Neuroblastoma Cells
Copyright policy )Subcellular Localization and Differentiation-Induced Redistribution of the Protein Tyrosine Phosphatase PTP-BL in Neuroblastoma Cells
1. In cells of epithelial origin the protein tyrosine phosphatase PTP-BL is predominantly localized at the apical membrane of polarized cells. This large submembranous multidomain PTP is also expressed in cells of neuronal origin. We studied the localization of PTP-BL in mouse neuroblastoma cells utilizing EGFP-tagged versions of the protein. 2. In proliferating Neuro-2a cells, immunofluorescence and immuno-electron microscopy revealed a submembranous FERM domain-dependent localization at cell-cell boundaries for EGFP-PTP-BL. Additionally, significant amounts of EGFP-PTP-BL are located in the cytoplasm as well as in nuclei. Upon serum depletion-induced differentiation of Neuro-2a cells, a partial shift of EGFP-PTP-BL from a cortical localization to cytoskeleton-like F-actin-positive structures is observed. Parallel biochemical studies corroborate this finding and reveal a serum depletion-induced shift of EFGP-PTP-BL from a membrane(-associated) fraction to an NP40-soluble cytoskeletal fraction. 3. Different pools of PTP-BL-containing protein complexes can be discerned in neuronal cells, reflecting distinct molecular microenvironments in which PTP-BL may exert its function.
- Radboud University Nijmegen Netherlands
Cytoplasm, Macromolecular Substances, DCN 2: Functional Neurogenomics, Green Fluorescent Proteins, Protein Tyrosine Phosphatase, Non-Receptor Type 13, N4i 1: Pathogenesis and modulation of inflammation, Culture Media, Serum-Free, Mice, Neuroblastoma, UMCN 5.3: Cellular energy metabolism, Cell Line, Tumor, Animals, NCEBP 2: Evaluation of complex medical interventions, Cells, Cultured, Cytoskeleton, Cell Nucleus, Neurons, Cell Membrane, Cell Differentiation, Actins, Cell Compartmentation, Protein Structure, Tertiary, Actin Cytoskeleton, Protein Transport, N4i 4: Auto-immunity, transplantation and immunotherapy, NCMLS 7: Chemical and physical biology
Cytoplasm, Macromolecular Substances, DCN 2: Functional Neurogenomics, Green Fluorescent Proteins, Protein Tyrosine Phosphatase, Non-Receptor Type 13, N4i 1: Pathogenesis and modulation of inflammation, Culture Media, Serum-Free, Mice, Neuroblastoma, UMCN 5.3: Cellular energy metabolism, Cell Line, Tumor, Animals, NCEBP 2: Evaluation of complex medical interventions, Cells, Cultured, Cytoskeleton, Cell Nucleus, Neurons, Cell Membrane, Cell Differentiation, Actins, Cell Compartmentation, Protein Structure, Tertiary, Actin Cytoskeleton, Protein Transport, N4i 4: Auto-immunity, transplantation and immunotherapy, NCMLS 7: Chemical and physical biology
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