CX 3 CR1 + CD8α + dendritic cells are a steady-state population related to plasmacytoid dendritic cells
Copyright policy )CX 3 CR1 + CD8α + dendritic cells are a steady-state population related to plasmacytoid dendritic cells
Lymphoid organs are characterized by a complex network of phenotypically distinct dendritic cells (DC) with potentially unique roles in pathogen recognition and immunostimulation. Classical DC (cDC) include two major subsets distinguished in the mouse by the expression of CD8α. Here we describe a subset of CD8α + DC in lymphoid organs of naïve mice characterized by expression of the CX 3 CR1 chemokine receptor. CX 3 CR1 + CD8α + DC lack hallmarks of classical CD8α + DC, including IL-12 secretion, the capacity to cross-present antigen, and their developmental dependence on the transcriptional factor BatF3. Gene-expression profiling showed that CX 3 CR1 + CD8α + DC resemble CD8α − cDC. The microarray analysis further revealed a unique plasmacytoid DC (PDC) gene signature of CX 3 CR1 + CD8α + DC. A PDC relationship of the cells is supported further by the fact that they harbor characteristic D–J Ig gene rearrangements and that development of CX 3 CR1 + CD8α + DC requires E2-2, the critical transcriptional regulator of PDC. Thus, CX 3 CR1 + CD8α + DC represent a unique DC subset, related to but distinct from PDC. Collectively, the expression-profiling data of this study refine the resolution of previous DC definitions, sharpen the border of classical CD8α + and CD8α − DC, and should assist the identification of human counterparts of murine DC subsets.
- Helmholtz Association of German Research Centres Germany
- University of Erlangen-Nuremberg Germany
- University of Mary United States
- Weizmann Institute of Science Israel
- Columbia University United States
Male, Mice, Knockout, Antigen Presentation, Mice, Inbred BALB C, CD8 Antigens, Gene Expression Profiling, Green Fluorescent Proteins, CX3C Chemokine Receptor 1, Dendritic Cells, Flow Cytometry, Immunophenotyping, Mice, Inbred C57BL, Mice, Basic-Leucine Zipper Transcription Factors, Receptors, HIV, Animals, Cell Lineage, Female, Receptors, Cytokine, Oligonucleotide Array Sequence Analysis
Male, Mice, Knockout, Antigen Presentation, Mice, Inbred BALB C, CD8 Antigens, Gene Expression Profiling, Green Fluorescent Proteins, CX3C Chemokine Receptor 1, Dendritic Cells, Flow Cytometry, Immunophenotyping, Mice, Inbred C57BL, Mice, Basic-Leucine Zipper Transcription Factors, Receptors, HIV, Animals, Cell Lineage, Female, Receptors, Cytokine, Oligonucleotide Array Sequence Analysis
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